Table 3.
Sterol composition according to their RP-HPLC profile of wild-type and cyp61 X.dendrorhous mutant strains (in mg/g dry yeast weight)
| |
Strains |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| UCD 67-385 | 385-cyp61(+/−) | 385-cyp61(−/−) | |||||||
|
Cultivation time (h) |
24 |
72 |
120 |
24 |
72 |
120 |
24 |
72 |
120 |
|
Ergosterol* |
4.74±0.53 |
3.10±0.09 |
2.24±0.42 |
3.19±0.48 |
2.87±0.32 |
2.91±0.34 |
ND |
ND |
ND |
|
Peak 2** |
0.23±0.03 |
0.030±0.003 |
0.10±0.05 |
0.62±0.05 |
0.11±0.03 |
0.12±0.02 |
6.34±2.68 |
2.36±0.74 |
2.39±0.27 |
|
Peak 3*** |
0.19±0.04 |
ND |
0.09±0.02 |
0.11±0.01 |
0.02±0.01 |
0.01±0.003 |
1.65±0.84 |
1.91±0.51 |
2.20±0.42 |
|
Total Sterols |
5.16±0.57 |
3.13±0.09 |
2.40±0.49 |
3.96±0.44 |
2.99±0.35 |
3.04±0.36 |
8.14±3.42 |
4.27±1.24 |
4.59±0.70 |
| |
Strains |
|
|
|
|||||
| |
CBS 6938 |
CBS-cyp61(−) |
|
|
|
||||
|
Cultivation time (h) |
24 |
72 |
120 |
24 |
72 |
120 |
|
|
|
|
Ergosterol* |
3.31±0.60 |
2.39±0.56 |
2.37±0.11 |
ND |
ND |
ND |
|
|
|
|
Peak 2** |
0.07±0.04 |
0.06±0.02 |
0.06±0.01 |
2.00±0.34 |
1.24±0.02 |
1.23±0.04 |
|
|
|
|
Peak 3*** |
0.03±0.001 |
0.02±0.01 |
0.03±0.01 |
2.38±0.29 |
2.60±0.08 |
3.05±0.17 |
|
|
|
|
Total Sterols |
3.45±0.56 |
2.41±0.59 |
2.46±0.11 |
4.38±0.61 |
3.85±0.1 |
4.28±0.21 |
|
|
|
| |
Strains |
|
|
|
|||||
| |
AVHN2 |
AV2-cyp61(−) |
|
|
|
||||
|
Cultivation time (h) |
24 |
72 |
120 |
24 |
72 |
120 |
|
|
|
|
Ergosterol* |
1.59±0.62 |
2.35±0.59 |
3.27±0.38 |
ND |
ND |
ND |
|
|
|
|
Peak 2** |
ND |
0.04±0.01 |
0.04±0.01 |
1.68±0.78 |
2.10±0.32 |
1.78±0.13 |
|
|
|
|
Peak 3*** |
ND |
ND |
ND |
1.39±0.82 |
2.27±0.18 |
2.39±0.52 |
|
|
|
| Total Sterols | 1.59±0.62 | 2.39±0.59 | 3.31±0.39 | 3.16±1.70 | 4.36±0.49 | 4.11±0.64 | |||
Table shows the mean values ± standard deviations of three independent experiments. Retention time: *: 18 min; **: 15 min; ***: 22 min. ND: Not detected.