Figure 6. The decreased level of H3 K27 tri-methylation leads to de-repression of Pref-1 gene. Non-targeting control siRNA (Ctrl) or EZH2-targeting siRNA (EZH2 KD) were transfected into C3H 10T1/2 cells and cells were collected after 2 d to examine: (A) EZH2 mRNA levels by RT-qPCR; (B) histone H3 K27 tri-methylation levels at the promoter region of Pref-1 gene by ChIP; (C) Pref-1 mRNA levels by RT-qPCR. These results are the averages of three independent experiments, and the error bars indicate standard deviations.