Figure 7. The increased levels of histone acetylation activate the transcription of PPARγ2 and aP2 genes. C3H 10T1/2 cells were treated with DMSO (Ctrl) or nicotinamide plus TSA (N + T) for 24 h before samples were collected for examination of: (A) histone H3 K9/14 acetylation and (B) histone H4 K12 acetylation levels at the promoter region of PPARγ2 and aP2 genes by ChIP; (C) PPARγ2 and (D) aP2 mRNA levels by RT-qPCR. These results are the averages of three independent experiments, and the error bars indicate standard deviations.