Figure 4. MiR-93 promotes cell survival and invasion. (A) The MT-1 cells transfected with miR-93 or mock (2.5 x 10⁵ cells per 1 mL) were seeded onto Petri dishes and tissue culture dishes and were maintained in a serum-free medium at 37°C and 5% CO₂ for 7 d. The cells were counted at the end of seventh day. The miR-93 transfected cells were seen to survive better. Error bars indicated standard deviation (SD) with n = 5. (B) The miR-93 and mock cells (1 x 10⁵) suspended in 100 μl serum-free medium were loaded onto the insert and incubated at 37°C for 48 h. The invasive cells were stained blue and were counted in six fields of views/membrane using a light microscope. Error bars indicate SD (n = 6). (C) MT-1 cells were transiently transfected with anti-miR-93 oligos or control oligos with random sequence. The cultures were maintained in tissue culture dishes in serum-free DMEM for 5 d, followed by microscopic examination and photographed. The number of cells was counted for statistical analysis. Error bars indicate SD (n = 6). (D) MT-1 cells were transiently transfected with anti-miR-93 oligos or control oligos with random sequence or with anti-miR-93 plasmid or control vector. The cells (1 x 10⁵) were subjected to invasion assays at 37°C for 64 h. Transfection with anti-miR-93 inhibited cell invasion.