Figure 5. Knockdown of Nek9 causes dissociation of γ-tubulin from spindle poles. Oocytes injected with Nek9 MO or control MO were cultured in fresh M2 medium for 8 h, followed by double-staining of α-tubulin (green), γ-tubulin (red) and DNA (blue). In the control MO-injected group, γ-tubulin was associated with the spindle poles at the metaphase I stage, whereas in the Nek9 MO-injected group, γ-tubulin dissociated from abnormal spindle poles and dispersed into the cytoplasm. α-tubulin, green; γ-tubulin, red; DNA, blue. Bar = 20 μm.