Figure 6. Nek9 depletion causes Pro-MI/MI arrest and failed PB1 extrusion and activation of SAC. (A) Oocytes of control MO and Nek9 MO groups were cultured for 12 h, followed by chromosome spreading experiments. (B) Percentages of the first polar body extrusion in the Nek9 MO injection group and control MO group. Data are presented as means +SEM of three independent experiments. Different superscripts indicate statistical difference at a p < 0.05 level of significance. (C) Detection of Bub3 in oocytes in Nek9 MO groups. Bub3, red; DNA, blue. Bar = 20 µm.