Figure 4. ER-α blocker suppresses cell proliferation in VHL-deficient cells. (A–C) Cell growth was estimated. Each cell line was treated with estrogen or Faslodex (5 μg/ml). Cells were maintained for maximum of 3 d, and cell numbers were calculated daily. (D) VHL-intact cell lines did not respond to estrogen or Faslodex. Each cell line was treated with estrogen or Faslodex for 6 h. After treatment, an immunoblot analysis was performed using the indicated antibodies. (E) The sensitivity to estrogen of Faslodex can be recovered by adding si-VHL to ACHN cells. Cell growth was estimated. After transfection with si-VHL, ACHN cells were treated with estrogen or Faslodex for 6 h. Cells were maintained for 4 d, and cell numbers were calculated daily.