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. 2013 Jan 23;8(1):e54636. doi: 10.1371/journal.pone.0054636

Figure 1. Kif6 expression in tissues, cells, mutant mice and structural information.

Figure 1

(A) Multi-tissue Western blot analysis of Kif6 protein expression. 20 µg of total protein from each tissue was separated by PAGE. The predicted molecular weight of Kif6 is 92KDa. An arrowhead indicates the Kif6 protein recognized by the C165 antisera. (B) Transfected c-myc epitope tagged Kif6a in HEK 293 cells and Kif6 in primary endothelial cells (HUVEC). (C) DNA sequence of exon 3 in Kif6 mutants, showing the A>T mutation at the −2 position of the exon 3 splice acceptor site. (D) RT-PCR of Kif6 RNA extracted from heart and brain tissue of wild type and mutant mice. (E) cDNA sequence of the mutant RT-PCR product, showing exon 2 reading into exon 4. (F) Ribbon representation of mouse KIF6 motor domain modeled on the crystal structure of the human KIF9 motor domain in complex with ADP (PDB 3nwn). The ADP molecule is represented in a sticks model and the region (residue K59 to residue S86) corresponding to exon 3 deletion is shown in red. Secondary structure elements are numbered according to the convention for the kinesin motors.