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. 2013 Jan 23;8(1):e54666. doi: 10.1371/journal.pone.0054666

Figure 7. IL-1β-dependent inhibition of the GABAergic transmission by activated microglia.

Figure 7

A) Representative image of immunostaining for IL-1β (green) on BV2 microglial cells (blue signal from DAPI) in basal condition (control) and after activation with Th1 pro-inflammatory cytokine mix. Control cells do not exhibit IL-1β staining, while the expression of the cytokine is visible in activated cells. Scale bar: 10 µm. B) Pooled (mean ± S.E.M) cumulative distributions of sIPSCs amplitude (left; bin size 10 pA) and inter-event interval (right; bin size 50 ms) recorded from neurons of C57BL/6 mice pre-incubated in non-activated (n = 12) and activated microglia (n = 12). Histograms in the insets are averages (mean ± S.E.M) of the corresponding median values. On top are trace records from typical neurons exposed to non-activated (left) or activated (right) microglia. C) Pooled (mean ± S.E.M) cumulative distributions of sIPSCs amplitude (left; bin size 10 pA) and inter-event interval (right; bin size 50 ms) recorded from neurons of C57BL/6 mice pre-incubated in activated microglia (n = 12) or activated microglia with the IL-1β receptor antagonist IL-1ra (10 µg/ml; n = 12). Histograms in the insets are averages (mean ± S.E.M) of the corresponding median values. On top are trace records from typical neurons exposed to activated microglia (left) or activated microglia with IL-1ra (right). ** and *** indicate p<0.01 and 0.001 t-test, respectively.