Figure 7. Epitope mapping of the cellular immune response in FIV infected.

T cell epitopes were mapped using spleen cells from 822,823,824,825,826,827 and 828. Analyses used 17 pools of 10 x 15-mer peptides, each overlapping by 10 amino acids (pools P1-P17). Representative ELISpots for spleen cells from animal 822 against peptide pools P6 to p10) are included. All animals were screened with 17 peptide pools and where strong responses were confirmed (*), individual epitopes were then identified by splitting pools into their component peptides and repeating the analyses.