Figure 5. Enzyme kinetics of UGT2A1-catalyzed glucuronidation of 6-hydroxyindole (A) and 4-MU (B), in the absence and presence of BSA.

The glucuronidation rates are presented as the average value ± S.E., and are expression level-normalized values. The concentrations of substrates were corrected for binding to 0.1% BSA. The determined enzyme kinetic parameters are presented in Table 3. See Materials and Methods for all further details.