Figure 6. Enzyme kinetics of UGT2B15-catalyzed glucuronidation of 17α-estradiol (A) and 4-MU (B), in the absence and presence of BSA.

The reaction with 17α-estradiol was analyzed for the formation of 17α-estradiol-3-β-D-glucuronide. The glucuronidation rates are presented as the average value ± S.E. These values are not expression normalized because we used commercial UGT2B15 without appropriate His-tag. The concentrations of substrates were corrected for binding to 0.1% BSA. The determined enzyme kinetic parameters are presented in Table 3. See Materials and Methods for all further details.