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Autophagy demonstrated little effects on cytotoxicity induced by MG132 in ovarian cancer cells. A, OVCAR3 were transfected with scramble shRNA or shRNA specific against Atg7 (shAtg7) for 24 h, then treated with 5 μM of MG132 for additional 24 h, and cell viability was measured using MTT assay. B, SKOV3, OVCAR3 or A2870 cells were treated with 5 μM of MG132 in the presence of chloroquine or bafilomycin A1 for 24 h, and cell viability was measured using MTT assay. N.S., p>0.05.
