Table 3.
Genes investigated | ND240 | HFD60 | HFD120 | HFD240 | HFDMET |
---|---|---|---|---|---|
GLUT-4 |
1.49±0.24 |
1.78±0.3bd |
2.41±0.42b |
2.05±0.32a |
1.13±0.13c |
Adiponectin |
1.56±0.15 |
0.81±0.37c |
1.13±0.62b |
1.68±0.30a |
1.66±0.24a |
RBP-4 | 0.10±0.13 | −1.20±0.16b | −1.30±0.44b | −1.63±0.33a | −1.50±0.17a |
Results are expressed as –fold variation over the appropriate control groups; ND240 indicate fold increase over ND, normal diet control group and HFD60, HFD120, HFD240 and HFDMET indicate fold increase over HFD, high-fat diet control group. Fold variations less than one were expressed as negative numbers (e.g., a –fold variation of 0.50 is expressed as −2.00). Values expressed are means ± S.D of triplicate measurements. Statistical significance was calculated based on the mean ΔCT values by DMRT for only mice fed with high-fat diet with or without GE. For same gene with different treatment groups, means in the different rows with different alphabets (a-d) were significantly different (p<0.05).