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. 2013 Jan 23;8(1):e53725. doi: 10.1371/journal.pone.0053725

Figure 5. Effects of DAPP on lipid peroxidation and regulatory endogenous antioxidant activities in Caco-2/15 cells.

Figure 5

Crude AB powder (250 µg/mL) and purified JC-047 fraction (250 µg/mL) were added to the apical compartment of differentiated Caco-2/15 cells for 24 h before incubation with Fe/Asc (200 µM/2 mM) and LPS (200 µg/mL) for 6 h at 37°C as described in Materials and Methods. Estimation of lipid peroxidation was assessed by measuring the MDA by HPLC (A). The activity of SOD (B), GPx (C) and G-Red (D) was then measured. Results represent the means ± SEM of n = 3 independent experiments. **P<0.01, ***P<0.001 vs. Ctrl; # P<0.05, ## P<0.01, ### P<0.001 vs. Fe/Asc; $$ P<0.01, $$$ P<0.001 vs. Fe/Asc+LPS.