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. 2013 Jan 23;8(1):e53725. doi: 10.1371/journal.pone.0053725

Figure 6. Effects of DAPP on oxidative stress or LPS-induced inflammation on inflammatory markers in Caco-2/15 cells.

Figure 6

Crude AB powder (250 µg/mL) and purified JC-047 fraction (250 µg/mL) were added to the apical compartment of differentiated Caco-2/15 cells for 24 h before incubation with Fe/Asc (200 µM/2 mM) and LPS (200 µg/mL) for 6 h at 37°C as described in Materials and Methods. Protein expression of the inflammatory markers TNF-α (A to C) and IL-6 (D to F) was determined by Western blot, respectively. Results represent the means ± SEM of n = 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. Ctrl; ### P<0.001 vs. Fe/Asc; † P<0.05, ††† P<0.001 vs. LPS; $$$ P<0.001 vs. Fe/Asc+LPS.