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. 2013 Jan 23;8(1):e53725. doi: 10.1371/journal.pone.0053725

Figure 7. Effects of DAPP on oxidative stress and LPS-induced inflammation on prostaglandin E2 in Caco-2/15 cells.

Figure 7

Crude AB powder (250 µg/mL) and purified JC-047 fraction (250 µg/mL) were added to the apical compartment of differentiated Caco-2/15 cells for 24 h before incubation with Fe/Asc (200 µM/2 mM) and LPS (200 µg/mL) (A), as well as indomethacin heptyl ester (0.4 µM) (B), as a selective cyclooxygenase (COX)-2 inhibitor, for 6 h at 37°C as described in Materials and Methods. PGE2 was determined by enzymatic immunoassay. Results represent the means ± SEM of N = 3 independent experiments. ***P<0.001 vs. Ctrl; ††† P<0.001 vs. LPS; $$$ P<0.001 vs Fe/Asc+LPS.