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Journal of Virology logoLink to Journal of Virology
. 1974 Feb;13(2):419–427. doi: 10.1128/jvi.13.2.419-427.1974

T4-Induced Activity Required for Specific Cleavage of a Bacteriophage Protein In Vitro

Judith Goldstein 1, Sewell P Champe 1
PMCID: PMC355312  PMID: 4589856

Abstract

We have examined the acid-soluble products formed during incubation of labeled substrate protein from T4-infected cells with unlabeled phage-infected cell extracts. If the substrate protein is prepared from cells infected with a T4 mutant blocked in cleavage of phage head precursor proteins, the products formed in vitro include a peptide indistinguishable by several criteria from one of the T4 internal peptides. Denatured as well as undenatured protein can serve as the substrate for the formation of this peptide. As expected, this peptide is not formed if protein from either uninfected cells or cells infected with wild-type T4 is used as substrate. The formation of this peptide in vitro is dependent on a factor present in extracts of phage-infected cells but absent from extracts of uninfected cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dickson R. C., Barnes S. L., Eiserling F. A. Structural proteins of bacteriophage T4. J Mol Biol. 1970 Nov 14;53(3):461–474. doi: 10.1016/0022-2836(70)90077-x. [DOI] [PubMed] [Google Scholar]
  2. Eddleman H. L., Champe S. P. Components in T4-infected cells associated with phage assembly. Virology. 1966 Nov;30(3):471–481. doi: 10.1016/0042-6822(66)90123-1. [DOI] [PubMed] [Google Scholar]
  3. Hosoda J., Cone R. Analysis of T4 phage proteins. I. Conversion of precursor proteins into lower molecular weight peptides during normal capsid formation. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1275–1281. doi: 10.1073/pnas.66.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hosoda J., Levinthal C. Protein synthesis by Escherichia coli infected with bacteriophage T4D. Virology. 1968 Apr;34(4):709–727. doi: 10.1016/0042-6822(68)90092-5. [DOI] [PubMed] [Google Scholar]
  5. Kellenberger E., Der Kamp C. K.-V. On a modification of the gene product P23 according to its use as subunit of either normal capsids of phage T4 or of polyheads. FEBS Lett. 1970 Jun 1;8(3):140–144. doi: 10.1016/0014-5793(70)80247-2. [DOI] [PubMed] [Google Scholar]
  6. Korant B. D. Cleavage of viral precursor proteins in vivo and in vitro. J Virol. 1972 Oct;10(4):751–759. doi: 10.1128/jvi.10.4.751-759.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Showe M. K., Black L. W. Assembly core of bacteriophage T4: an intermediate in head formation. Nat New Biol. 1973 Mar 21;242(116):70–75. doi: 10.1038/newbio242070a0. [DOI] [PubMed] [Google Scholar]
  9. Sternberg N., Champe S. P. Genetic determinant of an internal peptide of bacteriophage T4. J Mol Biol. 1969 Dec 28;46(3):377–392. doi: 10.1016/0022-2836(69)90183-1. [DOI] [PubMed] [Google Scholar]

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