Table 3. Overview of the amino acid changes in the 33 predicted sites of type II functional divergence.

Residue position (Human IκBα)	Amino acid change	Property change
40* [635]#	Q → K	Hydrophilic/+
44 [639]	H → Q	+/Hydrophilic
49 [644]	T → A	Hydrophilic/Hydrophobic
63 [660]	C → A	Hydrophilic/Hydrophobic
64 [661]	D → Q	−/Hydrophilic
73 [670]	N → R	Hydrophilic/+
79 [676]	A → C	Hydrophobic/Hydrophilic
80 [677]	C → A	Hydrophilic/Hydrophobic
117 [785]	L → C	Hydrophobic/Hydrophilic
119 [787]	S → V	Hydrophilic/Hydrophobic
122 [790]	G → H	Hydrophilic/+
123 [791]	Y → N	Hydrophilic/Hydrophilic
128 [823]	E → K	−/+
138 [833]	N → E	Hydrophilic/−
139 [834]	A → S	Hydrophobic/Hydrophilic
143 [859]	C → K	Hydrophilic/+
154 [870]	D → Q	−/Hydrophilic
155 [871]	L → E	Hydrophobic/−
156 [872]	Q → A	Hydrophilic/Hydrophobic
170 [891]	D → F	−/Hydrophobic
172 [893]	N → D	Hydrophilic/−
174 [895]	V → K	Hydrophobic/+
175 [896]	T → A	Hydrophilic/Hydrophobic
176 [897]	Y → H	Hydrophilic/+
179 [900]	Y → N	Hydrophilic/Hydrophilic
181 [902]	P → A	Hydrophilic/Hydrophobic
183 [904]	Q → H	Hydrophilic/+
185 [906]	T → A	Hydrophilic/Hydrophobic
187 [912]	G → R	Hydrophilic/+
194 [919]	Q → V	Hydrophilic/Hydrophobic
205 [930]	L → S	Hydrophobic/Hydrophilic
208 [933]	L → N	Hydrophobic/Hydrophilic
209 [934]	P → L	Hydrophilic/Hydrophobic

Sites of radical change were detected in pairwise type II functional analysis of vertebrate IκB taxonomic groups. The residue positions are provided based on the human IκBα reference sequence. The amino acid changes between subfamilies along with their property changes are provided.

+ represents positively charged amino acids; − represents negatively charged amino acids;

^*represents the residue position based on the human IκBα reference sequence;

^#represents the residue position in the full vertebrate IκB alignment.