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. 2013 Jan 23;8(1):e55151. doi: 10.1371/journal.pone.0055151

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© 2013 Miyamoto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–C, E: Anti-acetylated tubulin and anti-serotonin immunoreactivities. Serotonergic neural cell bodies are located on the lateral side of the giant axon (asterisks), and they project their axons medially (arrows). Anti-serotonin immunoreactivity was found in the dorsal nerve cords (arrowheads). D: Expression of syt was detected in the ventral nerve cord. F: Double-stain against anti-acetylated tubulin (green) and anti-FMRFamide (magenta) antibodies shows the presence of FMRFamidergic neurons in the ventral nerve cord. Scale bars = 100 µm. vcf, ventral ciliated field; vg, vestimental groove.