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(A, B) Immunostaining (A) and immunoblot (B) of nuclear-cytoplasm shuttling for FoxO1 with RGZ treatment and/or PPAR-γ overexpression. (C) Immunoblot for β-cell specific gene expressions and FoxO1 shuttling with insulin receptor RNAi. (D) Immunoblot for β-cell specific gene expressions after treatment with adenovirus containing rat FoxO1 shRNA.
