Fig. 4.

CGS21680, but not TNF-α or CGS21680+TNF-α, increases Rev-Erbα expression. THP-1 cells split at 12 pm were analyzed every 4 h starting at 12 am with quantitative real-time RT-PCR. Cells were kept in 10 % serum medium with a CGS21680 100 nM, b TNF-α 100 U/ml, and c CGS21680 100 nM+TNF-α 100 U/ml, or without the agonist and the cytokine (control), under strict cell growth monitoring. Results show the x-fold change for Rev-Erbα mRNA variations from time 0 (t=0). Data represent mean±SEM of four independent experiments. Statistics was performed by ANOVA followed by Newman–Keuls posttest, control vs CGS21680 *p<0.05.