. Author manuscript; available in PMC: 2014 Jan 23.

Published in final edited form as: ACS Appl Mater Interfaces. 2012 Dec 28;5(2):351–359. doi: 10.1021/am302285n

Table 1.

Characterization of Oligonucleotide Arrays Fabricated on Film-Coated Substrates^a

	Fluorescence Intensity		Signal-to-Noise

	FAM	Cy3	FAM	Cy3
Glass	4.5×10⁴ ± 5.7×10³	4.4×10⁴ ± 6.4×10³	96	63
PET	2.6×10⁴ ± 2.3×10³	4.7×10⁴ ± 5.4×10³	28	17
Heat-shrink (Prior to Shrinking)	3.4×10⁴ ± 5.1×10³	–	13	–
Heat-shrink (After Shrinking)	4.7×10⁴ ± 8.8×10³	–	15	–

Oligonucleotide arrays spots containing Probes 1 or 2 were fabricated on glass, PET, or heat-shrinkable polyolefin substrates coated with PEI/PVDMA multilayer films (see text). Average fluorescence signal and average signal-to-noise values were determined after hybridization using fluorescently labeled Complements 1 and 2. See Materials and Methods for additional information regarding determination of background signal and calculation of S/N ratios.