Table 1.
Fluorescence Intensity | Signal-to-Noise | |||
---|---|---|---|---|
FAM | Cy3 | FAM | Cy3 | |
Glass | 4.5×104 ± 5.7×103 | 4.4×104 ± 6.4×103 | 96 | 63 |
PET | 2.6×104 ± 2.3×103 | 4.7×104 ± 5.4×103 | 28 | 17 |
Heat-shrink (Prior to Shrinking) | 3.4×104 ± 5.1×103 | – | 13 | – |
Heat-shrink (After Shrinking) | 4.7×104 ± 8.8×103 | – | 15 | – |
Oligonucleotide arrays spots containing Probes 1 or 2 were fabricated on glass, PET, or heat-shrinkable polyolefin substrates coated with PEI/PVDMA multilayer films (see text). Average fluorescence signal and average signal-to-noise values were determined after hybridization using fluorescently labeled Complements 1 and 2. See Materials and Methods for additional information regarding determination of background signal and calculation of S/N ratios.