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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Dev Dyn. 2013 Feb;242(2):108–121. doi: 10.1002/dvdy.23906

Figure 4. HDAC inhibition prevents hematopoietic induction in ectodermal explants downstream of bmp4 and gata1.

Figure 4

A-F) Expression of bmp-4 (A, B), chordin (C,D), and noggin (E,F) in late gastrula stage embryos is unaffected by VPA (B, D, F); orientation: posterior view, dorsal to the top. G-I) Quantitative RT-PCR of ectodermal explants harvested at tailbud stages (stage 28-31). G) Injection of bmp4 mRNA (2 ng) induced globin expression and VPA inhibited this effect. H) Injection gata1 mRNA (200pg) induced globin expression and VPA treatment inhibited this effect. I) Injection of bmp4 induced expression of runx1 and VPA blocked this effect. J) VPA upregulated endogenous expression of ncam in uninjected explants.