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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Dev Dyn. 2013 Feb;242(2):108–121. doi: 10.1002/dvdy.23906

Figure 7. Runx1 expression rescues VPA-mediated hematopoietic defects.

Figure 7

A) Benzidine staining of hemoglobin (blue) in untreated stage 43 tadpole. B) Treatment with VPA inhibits RBC development. C) Injection of runx1 mRNA partially recovered erythrocyte development in VPA treated embryos. D) Percentage of benzidine positive tadpoles for uninjected (n=20), VPA-treated (n=25), VPA-treated + runx1 (n=21), and runx1 expression alone (n=21). This experiment was repeated with 4 separate clutches of embryos and the table shows data from all 4 experiments. Intensity of benzidine stain was reduced in VPA and VPA+runx1 groups (as shown in panel C). E-G) Expression of globin was detected by WISH in all (23/23) control tadpoles, 0/20 VPA-treated tadpoles, and 7/18 VPA-treated, runx1-expressing tadpoles.