Table 1.

Expression of R-spondin family members during development of the cochlea. Tabulated results of an RT PCR assay showing that only Rspo2 is expressed throughout development. Trace amounts of Rspo3 were detected at E17.5 and P0 - a time after cell fate specification has occurred in the organ of Corti. Rspo1 and 4 were not detected at any stage. Positive and negative controls (kidney, lung or digits) were chosen based on previous expression analysis screens (Nam et al. 2007b).

	E14.5 Kidney	E14.5 Lung	E15.5 Digits	E13.25 cochlea	E15.5 cochlea	E17.5 cochlea	P0 cochlea
Rspo1	Yes	No		No	No	No	No
Rspo2	No	Yes		Yes	Yes	Yes	Yes
Rspo3	Yes	No		No	No	Trace	Trace
Rspo4		No	Yes	No	No	No	No