Figure 4.

SDR controls body growth through InR signaling in vivo. (A) dFoxo localization was analyzed in the fat body of mid-third instar larvae. Early third instar larvae just after molting were grown on normal food conditions (normal food) or adverse food conditions (10% food contents) for 24 h. Tub-Gal4 was used for the manipulation of SDR levels. Bars, 25 μm. (B) Thor/4EBP, InR, and dilp6 transcripts were analyzed by qRT–PCR in the sample of A. The values are the mean and SD (n = 3–4). (C) SDR genetically interacts with InR signaling in vivo. The volume of pupae was analyzed in flies of the indicated genotypes. The values are the mean and SEM (n = 40). (*) P < 0.05; (**) P < 0.01 (Student's t-test).