Fig. 5.

Thapsigargin's effect on SH-SY5Y cell ECAR values. For injection A, SH-SY5Y cells received either 0 μM (red line and red bar), 0.3 μM (blue line and blue bar), or 3 μM (cyan line) thapsigargin. For injection B, all of the cells received 100 μM carbachol, for injection C all cells received 0.3 μM FCCP, and for injection D all cells received 1 μM rotenone and 2 μM myxothiazol. Point 4 represents the “baseline” value; the horizontal line drawn through point 4 depicts this value (100% of the baseline value) across the horizontal length of the graph. A) Both 0.3 μM and 3 μM thapsigargin reduced the ECAR below its baseline level. Carbachol subsequently increased the ECAR in the 0 μM thapsigargin and 0.3 μM thapsigargin groups, but carbachol did not increase the ECAR in the group that received 3 μM thapsigargin. B) In the 0.3 μM thapsigargin group carbachol treatment brought the ECAR back above the pre-thapsigargin baseline, but at time point 10 the percent increase over baseline (time point 4) was less (a 10% increase) than it was when no thapsigargin was present (a 26% increase).