Fig. 2.

The influence of PSGL1 addition on the physicochemical and biological activity of liposomes. (a) Dynamic light scattering analysis of the size and polydispersity of liposomes or modified liposomes. (b) Ability of Fc-control or PSGL1 liposomes to adhere to E-selectin coated plates, as measured by detection of luminescence in wells after incubation with luciferin loaded liposomes, washing, rupture of liposomes and luciferase enzyme addition. N = 3, SD shown, significance (p < 0.05) calculated by ANOVA. (c) Ability of liposomes to adhere to / deliver luciferin to HUVECs expressing the luciferase enzyme, as assayed after incubation with luciferin loaded liposomes, washing, and multiple subsequent luminescence measurements. Black line and diamonds = unmodified liposomes, blue line and squares = Fc control liposomes, red line and triangles = PSGL1 liposomes. N = 4, standard error (se) shown, significance (p < 0.001) calculated by ANOVA with Tukey post test all samples comparison using PRISM.