Figure 3. Two-photon fluorescence imaging of cortical pyramidal neurons with 910-nm and 1030-nm excitation in an adult H-line mouse brain.

(a) Maximum intensity projections of three-dimensional stacks were obtained with 910-nm and 1030-nm lasers (b). Four normalized xy frames from the z-stack at various depths including the dura matter, apical dendrite, soma, and white matter, respectively (left panels). The arrowhead shows a part of a basal dendritic process at cortex layer V.