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. 2013 Jan 24;3:1129. doi: 10.1038/srep01129

Figure 1. Identification of the Entamoeba TOM complex and its novel subunit.

Figure 1

(a), The TOM complex demonstrated by BN-PAGE and immunoblot analysis. (b), Immunoprecipitation of the TOM complex (arrows) from Tom40-HA transformant by BN-PAGE and immunoblot analysis with anti-HA antibody. (c), SDS-PAGE and silver stain of immunoprecipitated TOM complex from Tom40-HA. Arrowhead indicates Tom60. (d), Prediction of the secondary structure and the domain organization of EhTom60. Gray box indicates the hydrophobic cluster, while pink and yellow boxes depict putative tetratricopeptide repeats (TPRs) conserved among genus Entamoeba or those specific to E. histolytica, respectively. Probability and E-value are shown (Supplementary Fig. S4). Green and blue boxes indicate α-helices and β-strands, respectively, predicted by PSIPRED (Supplementary Fig. S6).