Step |
Notes |
1 |
Dispensing with the FlexDrop IV. (SET7/9 at 300 nM; SET8 at 3 μM; SETD2 at 500 nM; EuHMTase1 at 20 nM) |
2 to 4 |
Dispensing on the PP-384-M Personal Pipettor using a custom 384 head. |
5 |
Plates incubated at room temperature. |
6 |
Dispensing with the FlexDrop IV. (1.5 μM [3H-Me]-SAM and 3 μM Peptide) |
7 |
Plates incubated at room temperature. (SET7/9 for 3h; SET8 for 8h; SETD2 for 4h; EuHMTase1 for 1h) |
8 |
Dispensing with the Multidrop384. |
9 |
Plates incubated at room temperature. |
10 |
Plates centrifuged at 2,000 rpm for 1 min. |
11 |
Radiometric signal quantified with empty epi mirror and empty emission mirror. |