| Step | Notes |
| 1 | Dispensing with the FlexDrop IV. (SET7/9 at 300 nM; SET8 at 3 μM; SETD2 at 500 nM; EuHMTase1 at 20 nM) |
| 2 to 4 | Dispensing on the PP-384-M Personal Pipettor using a custom 384 head. |
| 5 | Plates incubated at room temperature. |
| 6 | Dispensing with the FlexDrop IV. (1.5 μM [3H-Me]-SAM and 3 μM Peptide) |
| 7 | Plates incubated at room temperature. (SET7/9 for 3h; SET8 for 8h; SETD2 for 4h; EuHMTase1 for 1h) |
| 8 | Dispensing with the Multidrop384. |
| 9 | Plates incubated at room temperature. |
| 10 | Plates centrifuged at 2,000 rpm for 1 min. |
| 11 | Radiometric signal quantified with empty epi mirror and empty emission mirror. |