Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Comb Chem High Throughput Screen. 2012 Jun 1;15(5):359–371. doi: 10.2174/138620712800194468
Step Notes
1 Dispensing with the FlexDrop IV. (SET7/9 at 300 nM; SET8 at 3 μM; SETD2 at 500 nM; EuHMTase1 at 20 nM)
2 to 4 Dispensing on the PP-384-M Personal Pipettor using a custom 384 head.
5 Plates incubated at room temperature.
6 Dispensing with the FlexDrop IV. (1.5 μM [3H-Me]-SAM and 3 μM Peptide)
7 Plates incubated at room temperature. (SET7/9 for 3h; SET8 for 8h; SETD2 for 4h; EuHMTase1 for 1h)
8 Dispensing with the Multidrop384.
9 Plates incubated at room temperature.
10 Plates centrifuged at 2,000 rpm for 1 min.
11 Radiometric signal quantified with empty epi mirror and empty emission mirror.