Fig 1.

Effects of PZQ and ABZSO on in vitro release/secretion of AP and PGI by T. crassiceps. T. crassiceps metacestodes were cultured in vitro in the indicated concentrations of PZQ (A and C) or ABZSO (B and D) for 6 days. Supernatants collected (and replaced) every 24 h were tested for AP concentrations (A and B) or PGI activity (C and D) (see Materials and Methods). Data are shown as percentages of untreated parasites at the corresponding culture time point (A) or as optical density at 340 nm (OD₃₄₀) corrected for wells with no drug (medium alone) and are representative of three (repeated) experiments. Molar concentrations of drugs provided in the graph legends correspond to 10 ng/ml, 50 ng/ml, and 100 ng/ml for PZQ and 10 ng/ml, 50 ng/ml, 100 ng/ml, and 500 ng/ml for ABZSO. Significant differences from untreated cysts are indicated by asterisks (*, P < 0.05; **, P < 0.01; ***, P < 0.005).