Fig 5.

Real-time PCR verification of microarray analysis. The deletion of CgSTB5 resulted in the upregulation of CgCDR1, CgPDH1, and CgYOR1, all members of the ABC transporter family of proteins, as well as CgPDR1, the transcriptional regulator of ABC transporters (upper panel). The same effect was seen in the Δpdr1 background but at a lower magnitude (lower panel). Overexpression of CgSTB5 did not significantly decrease transcription of these transporters despite increasing azole susceptibility. The standard error of the mean is shown for each gene.