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. 2013 Feb;57(2):959–967. doi: 10.1128/AAC.01278-12

Table 3.

Primers and probes used in this study (grouped by application)

Primer Sequence (5′–3′)
Plasmid construction primers
    CgSTB5S GCATGGATCCATGAGTGGCCCAGATAAGGG
    CgSTB5AS GCATGAATTCTTATCTAAAGCGATCCTCCCA
Deletion cassette primers
    CgSTB5DS1 TCATATGTCATCATAGCCTACTTGACTAGGAGTTATAACAGATATATTAATAGAGTAGGATTTACAAGGATTTCACTATGTCGAAAGCTACATATAAGGA
    CgSTB5DAS2 CACAATTATTGACAAGTTTCAGTTTCATAATCCGTCTGCATCTTCATTGATATCATCCTGGGGCTTACTTCTTATCTTATTAGTTTTGCTGGCCGCATCT
    CgSTB5DS3 CAAGCTAGTGATCAGATAAAACTGGGGAGTTTAATCAACAAGAGTTTTTAGCCCTTTCTTAGTCCCAATCCAACTGAATCATATGTCATCATAGCCTACT
    CgSTB5DAS4 TCTTGTGCTATACGGCAGAAACATAAATAGATTAAGATAAACTATTAGCACTAATCGGAATAAAGGGAACTGAATAGCACAATTATTGACAAGTTTCAGT
    CgPDR1D1 TGGAATTAGTGTTTTATTCTGCCTTTTTTTTTAGAATATATTGGTAAAGTCATTCTTTAGCTACGTTATTGAGAGAATATGTCGAAAGCTACATATAAGG
    CgPDR1D2 GCAACATAACCACTAACAAATGATTTTTCAGGTTAAATATAAAATTATACAGGCTATGCACACTGTCTAAATTAATAGCATTAGTTTTGCTGGCCGCATC
    CgPDR1D3 TTATTACTTAAACAATTTTTAAGTAACACATTCAAACTTCCATTACTTCGTACCCCATATCGTATTGCCATTGTGATATGGAATTAGTGTTTTATTCTGC
    CgPDR1D4 ATTAAACTACAATTTTTTAATGAGAGATATTGTAGTGTTATCGCTAATTTGAGGTAGTCTAAGTCTCATGTAAAATTATGCAACATAACCACTAACAAAT
qRT-PCR primers
    CgACT1F TTGGACTCTGGTGACGGTGTTA
    CgACT1R AAAATAGCGTGTGGCAAAGAGAA
    CgCDR1F AGATGTGTTGGTTCTGTCTCAAAGAC
    CgCDR1R CCGGAATACATTGACAAACCAAG
    CgPDH1F AATGGATGTTAGAAGTAGTTGGAGCAG
    CgPDH1R TGTTCGGAATTTCTCCACACCT
    CgYOR1F CGCTGGGAAGGCCAAGA
    CgYOR1R CTCCCCGGACGTCAGAATAG
    CgPDR1F AACGATTATTCAATTGCAACAACG
    CgPDR1R CCTCACAATAAGGAAAGTCTGCG
qRT- PCR probes
    CgACT1 CCACGTTGTTCCAATTTACGCCGG
    CgCDR1 TTATCTGCTGCGATGGTTCCTGCTTCC
    CgPDH1 CAGGCTCACATGCAAACCAAGACTACCAT
    CgYOR1 CTCGCCGGTGCAGGATTACGATCTAGA
    CgPDR1 TCGAATATTATGCACCATCATGTCTGTGTTTAGCT