. 2013 Feb;57(2):959–967. doi: 10.1128/AAC.01278-12

Table 3.

Primers and probes used in this study (grouped by application)

Primer	Sequence (5′–3′)
Plasmid construction primers
CgSTB5S	`GCATGGATCCATGAGTGGCCCAGATAAGGG`
CgSTB5AS	`GCATGAATTCTTATCTAAAGCGATCCTCCCA`
Deletion cassette primers
CgSTB5DS1	`TCATATGTCATCATAGCCTACTTGACTAGGAGTTATAACAGATATATTAATAGAGTAGGATTTACAAGGATTTCACTATGTCGAAAGCTACATATAAGGA`
CgSTB5DAS2	`CACAATTATTGACAAGTTTCAGTTTCATAATCCGTCTGCATCTTCATTGATATCATCCTGGGGCTTACTTCTTATCTTATTAGTTTTGCTGGCCGCATCT`
CgSTB5DS3	`CAAGCTAGTGATCAGATAAAACTGGGGAGTTTAATCAACAAGAGTTTTTAGCCCTTTCTTAGTCCCAATCCAACTGAATCATATGTCATCATAGCCTACT`
CgSTB5DAS4	`TCTTGTGCTATACGGCAGAAACATAAATAGATTAAGATAAACTATTAGCACTAATCGGAATAAAGGGAACTGAATAGCACAATTATTGACAAGTTTCAGT`
CgPDR1D1	`TGGAATTAGTGTTTTATTCTGCCTTTTTTTTTAGAATATATTGGTAAAGTCATTCTTTAGCTACGTTATTGAGAGAATATGTCGAAAGCTACATATAAGG`
CgPDR1D2	`GCAACATAACCACTAACAAATGATTTTTCAGGTTAAATATAAAATTATACAGGCTATGCACACTGTCTAAATTAATAGCATTAGTTTTGCTGGCCGCATC`
CgPDR1D3	`TTATTACTTAAACAATTTTTAAGTAACACATTCAAACTTCCATTACTTCGTACCCCATATCGTATTGCCATTGTGATATGGAATTAGTGTTTTATTCTGC`
CgPDR1D4	`ATTAAACTACAATTTTTTAATGAGAGATATTGTAGTGTTATCGCTAATTTGAGGTAGTCTAAGTCTCATGTAAAATTATGCAACATAACCACTAACAAAT`
qRT-PCR primers
CgACT1F	`TTGGACTCTGGTGACGGTGTTA`
CgACT1R	`AAAATAGCGTGTGGCAAAGAGAA`
CgCDR1F	`AGATGTGTTGGTTCTGTCTCAAAGAC`
CgCDR1R	`CCGGAATACATTGACAAACCAAG`
CgPDH1F	`AATGGATGTTAGAAGTAGTTGGAGCAG`
CgPDH1R	`TGTTCGGAATTTCTCCACACCT`
CgYOR1F	`CGCTGGGAAGGCCAAGA`
CgYOR1R	`CTCCCCGGACGTCAGAATAG`
CgPDR1F	`AACGATTATTCAATTGCAACAACG`
CgPDR1R	`CCTCACAATAAGGAAAGTCTGCG`
qRT- PCR probes
CgACT1	`CCACGTTGTTCCAATTTACGCCGG`
CgCDR1	`TTATCTGCTGCGATGGTTCCTGCTTCC`
CgPDH1	`CAGGCTCACATGCAAACCAAGACTACCAT`
CgYOR1	`CTCGCCGGTGCAGGATTACGATCTAGA`
CgPDR1	`TCGAATATTATGCACCATCATGTCTGTGTTTAGCT`