Fig 1.

(A) Replacement of the M. tuberculosis mmr gene. An allelic exchange substrate (AES) for replacement of mmr (Rv3065) was generated by cloning 576-bp upstream and 996-bp downstream regions on either side of the hyg resistance cassette of pYUB854. The AES was obtained by PCR or restriction digestion of this segment; (B) integrative plasmid pCRS5 that contains a copy of mmr and that was used to complement the Mmr knockout mutant; (C) replicative plasmid pCVZ2 used to overexpress mmr in H37Rv.