Table 3.
M. tuberculosis strain | MIC (μg/ml)d |
|||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Ethidium bromide |
Tetraphenylphosphonium |
Acriflavine |
Safranin O |
CTAB |
||||||||||||||||
No inhibitor | CCCP | CPZ | VP | No inhibitor | CCCP | CPZ | VP | No inhibitor | CCCP | CPZ | VP | No inhibitor | CCCP | CPZ | VP | No inhibitor | CCCP | CPZ | VP | |
H37Rv | 2 | 2 | 2 | 0.5 | 12.5 | 3.1 | 12.5 | 12.5 | 3 | 1.5 | 3 | 3 | 1 | 0.5 | 1 | 0.5 | 12.5 | 3.1 | 12.5 | 12.5 |
MmrKOa | 1 | 1 | 1 | 0.13 | 3.1 | 3.1 | 1.5 | 1.5 | 3 | 1.5 | 3 | 1.5 | 0.5 | 0.5 | 0.25 | 0.5 | 6.25 | 3.1 | 6.25 | 6.25 |
MmrKO::pCRS5b | 2 | 2 | 2 | 1 | 12.5 | 3.1 | 12.5 | 12.5 | 3 | 1.5 | 3 | 3 | 1 | 0.5 | 1 | 0.5 | 12.5 | 3.1 | 12.5 | 12.5 |
H37Rv::pCVZ2c | 4 | 2 | 4 | 1 | 12.5 | 6.2 | 12.5 | 12.5 | 6 | 0.18 | 6 | 6 | 2 | 0.06 | 2 | 2 | 12.5 | 0.4 | 12.5 | 12.5 |
M. tuberculosis H37Rv with mmr gene inactivated.
MmrKO complemented with pCRS5.
M. tuberculosis H37Rv containing pCVZ2.
CCCP, carbonyl cyanide m-chlorophenylhydrazone; CPZ, chlorpromazine; CTAB, cetyltrimethylammonium bromide; VP, verapamil. Data in bold represent at least a 4-fold reduction, considered to denote a significant MIC reduction in the presence of an efflux inhibitor.