Fig 2.

Inhibition of HCV1b RNA replication by DBPR110. DBPR110 inhibits the HCV replication stages (24 to 72 h) rather than the viral translation stages (4 to 8 h). The HCV1b replicon was electroporated into Huh-7.5 cells, which were then maintained in the absence (DMSO) or presence of 10 or 100 pM DBPR110. Renilla luciferase (Rluc) activity was monitored at the indicated time points posttransfection. The numbers above the DBPR110-treated time points represent the percentages of luciferase signals relative to the DMSO-treated controls (100%), and the data are presented as the means ± SD of three independent experiments. ***, P < 0.001, compared with the DMSO group. Statistical significance was calculated by using an unpaired Student t test as described in Materials and Methods.