Fig 2.
In vivo bioluminescent signals of CA-MRSA-infected wounds highly correlate with ex vivo bacterial CFU counts. Scalpel wounds on the backs of mice were inoculated with USA300 LAC::lux (2 × 106 CFU/10 μl). Correlation between in vivo bioluminescent signals (mean total flux [photons/s] ± the SEM) (logarithmic scale) from mice (n = 6 per group) imaged at 4 h and on days 1, 3, 5, and 7 and total ex vivo CFU (logarithmic scale) recovered from 8-mm lesional punch biopsies performed on euthanized mice (n = 6 per group) at the same time points. The trendline and the correlation coefficient of determination (R2) between in vivo bioluminescent signals and total CFU are shown.