Table 2.

List of selected genes whose transcription is affected by niclosamide^a

Gene role and PA no.	Gene name	Fold changeb	Gene product
Virulence factors
PA0026	plcB	−4.15	Phospholipase C, PlcB
PA0070	tagQ1	2.73	Protein secretion apparatus, type VI secretion system
PA0085	hcp1	2.99	Type VI protein secretion system component Hcp
PA1871	lasA	−10.05	LasA protease precursor
PA1901	phzC1, phzC2	−2.22	Phenazine biosynthesis protein PhzC
PA1905	phzG2	−2.19	Probable pyridoxamine 5′-phosphate oxidase
PA2300	chiC	−7.24	Chitinase
PA3478	rhlB	−5.99	Rhamnosyltransferase chain B
PA3479	rhlA	−5.65	Rhamnosyltransferase chain A
PA3724	lasB	−2.90	Elastase LasB
PA4210	phzA1, phzA2	−3.06	Probable phenazine biosynthesis protein
PA4211	phzB1, phzB2	−2.42	Probable phenazine biosynthesis protein
Adhesion and biofilm formation
PA0852	cbpD	−3.92	Chitin-binding protein CbpD precursor
PA1120	tpbB	−2.19	Diguanylate cyclase
PA2570	pa1L	−4.08	PA-I galactophilic lectin
PA2572		−3.17	Probable two-component response regulator
PA4781		−2.50	Cyclic di-GMP phosphodiesterase
Gene regulation
PA2931	cifR	2.10	Transcriptional regulator
PA3477	rhlR	−2.56	Transcriptional regulator RhlR
PA4296	pprB	−3.21	Two-component response regulator
Drug efflux
PA4205	mexG	−29.17	Hypothetical protein
PA4206	mexH	−16.94	Probable RND efflux membrane fusion protein precursor
PA4207	mexI	−13.87	Probable RND efflux transporter
PA4208	opmD	−9.12	Probable outer membrane protein precursor

^aThe PA number, gene name, and gene product are from the Pseudomonas Genome Database. Genes previously reported to be activated by the 3OC₁₂-HSL and/or C₄-HSL QS systems are shown in bold characters (38–40). RND, resistance-nodulation-cell division.

^bFold change in gene expression of P. aeruginosa PA14 grown in LB supplemented with 20 μM niclosamide compared to the same strain grown in LB.