Table 3.

Susceptibility of wild-type and NS5b site II mutant HCV GT 1b replicons to GS-9669 and other reference NS5B inhibitors

Compound	Mean EC50 (nM) for WT (GT 1b) ± SDa	Fold resistanceb against NS5B mutant
		M423T	M423I	M423V	I482L	R422K	L419M
GS-9669	11 ± 1.9	3.4	7.2	12	38	36	19
Lomibuvir	11 ± 3.0	81	40	33	105	105	24
Filibuvir	80 ± 28	37	31	37	17	NT	NT
2′-C-Me-A	206 ± 86	0.8	0.7	0.4	0.6	0.7	1.2

^aArithmetic means of data from at least two experiments Values were generated in a 96-well assay manual format. WT, wild type.

^bFold resistance is calculated as the ratio of mutant EC₅₀ to wild-type EC₅₀ values. NT, not tested.