Table 1.
List of genesa whose loss confers resistance to 0.8-nm AuTMAT
| Locus | Gene name (no. of times mutant was isolated) | Function (Saccharomyces Genome Database 25 December 2011) | RDb |
|---|---|---|---|
| YBR268w | MRPL37 (2) | Mitochondrial ribosomal protein of the large subunit | Yes |
| YCR046c | IMG1 (1) | Mitochondrial ribosomal protein of the large subunit; required for respiration and maintenance of mitochondrial genome | Yes |
| YER114c | BOI2 (1) | Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain | Yes |
| YGL166w | CUP2 (1) | Cu-binding transcription factor; activates transcription of CUP1-1 and CUP1-2 metallothionein genes in response to elevated Cu concentrations | No |
| YGR150c | CCM1 (2) | Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has pentatricopeptide repeat (PPR) motifs; mutant has defective plasma membrane electron transport | Yes |
| YGR207c | CIR1 (4) | Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may play role in oxidative stress response | No |
| YHR006w | STP2 (6) | Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes | Yes |
| YJL129c | TRK1 (1) | Component of Trk1p-Trk2p K transport system; 180-kDa high-affinity K transporter; phosphorylated in vivo and interacts physically with phosphatase Ppz1p, suggesting Trk1p activity is regulated by phosphorylation | No |
| YLR382c | NAM2 (6) | Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance | Yes |
| YLR439w | MRPL4 (1) | Mitochondrial ribosomal protein of the large subunit; homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit | Yes |
| YMR072w | ABF2 (1) | Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination; member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation | Yes |
| YMR155w | (1) | Unknown | No |
| YMR173w | DDR48 (1) | DNA damage-responsive protein; expression increases in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of amino acid sequence NNNDSYGS | No |
| YMR192w | GYL1 (1) | Putative GTPase-activating protein (GAP) with role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p; colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p | No |
| YMR223w | UBP8 (1) | Ubiquitin-specific protease; member of SAGA (Spt-Ada-Gcn5-acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2 | No |
| YPL183w-A | RTC6 (1) | Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36; may be a mitochondrial ribosomal protein encoded in the nucleus | Yes |
| YPR100w | MRPL51 (1) | Mitochondrial ribosomal protein of the large subunit | Yes |
a
Eight of the 17 gene deletions were confirmed independently to be responsible for resistance. Five deletion mutants were crossed to deletion strains of the opposite mating type to generate homozygous diploids that exhibited the same phenotype (GYL1Δ, ABF2Δ, STP2Δ, NAM2Δ, BOI1Δ mutants). Two were transformed with wild-type alleles of the deleted genes, which restored sensitivity (DDR48, CIR1). One deletion mutant obtained in a different strain background was also found to be resistant (TRK1Δ mutants).