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. 2013 Jan;79(2):460–468. doi: 10.1128/AEM.02551-12

Fig 3.

Fig 3

Bmp1 enhanced the activity of crystal protein Cry5Ba against C. elegans. We conducted quantitative growth, mortality tests, and brood size assays at the condition of 20°C and pH 7.0 to test the toxicity of crystal proteins against nematodes. (A) Mortality assay using different concentrations of each protein (Cry5Ba, Cry5Ba-Bmp1 mixtures, and Bmp1) against synchronized L4 nematodes. Error bars represent the standard deviations from the mean averages over three independent experiments. (B) Brood size assay using different concentrations of each protein (Cry5Ba, Cry5Ba-Bmp1 mixtures, and Bmp1) against synchronized L4 nematodes. Error bars represent the standard deviations from mean averages over three independent experiments. (C) Growth assay using different concentrations of each protein (Cry5Ba, Cry5Ba-Bmp1 mixtures, and Bmp1) against synchronized L4 nematodes. The quantitative growth of crystal proteins against nematodes was tested by photograph at least 30 worms for each condition at ×100 magnification on a compound microscope.