Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristicsa | Origin or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44 ΔlacU1169(ϕlacZΔ)hsdR17 recA1 endA1 gyrA96 Δthi relA1 | –b |
| BL21(DE3) | T7 promoter | – |
| EMB0717 | pEMB0717 (contains bmp; Kanr) | This study |
| Bacillus thuringiensis | ||
| YBT-1518 | Wide type, containing the cry55Aa, cry6Aa, and cry5Ba genes | 14 |
| BMB0215 | Derivative of YBT-1518 (containing cry5Ba; Ermr) | 14 |
| BMB31 | Derivative of BMB171 (containing cry1Ac10; Ermr) | 8 |
| Plasmids | ||
| pET28a | ori E. coli (Kanr), 5.4 kb | – |
| pEMB0717 | Derivative of pET28a (containing the bmp gene; Kanr) | This study |
a
Ermr, erythromycin resistance; Kanr, kanamycin resistance.
b
–, stored at State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.