Table 3.

Effect of protease inhibitors on Bmp1 protein

Inhibitor	Mean remaining activity (%) ± SDa
	0 mM	1 mM	4 mM	8 mM	10 mM
EDTA	100	90.48 ± 1.86	53.57 ± 1.16	59.76 ± 1.13	49.29 ± 1.84
PMSF	100	64.97 ± 2.81	56.80 ± 2.33	51.20 ± 3.56	26.68 ± 1.15
Pepstatin A	100	86.31 ± 2.93	84.52 ± 5.93	96.43 ± 3.49	105.36 ± 7.19
Zn2+	100	106.00 ± 4.60	72.00 ± 2.91	38.00 ± 2.52	43.00 ± 2.86
Ca2+	100	80.00 ± 6.88	73.00 ± 3.86	63.00 ± 10.07	51.00 ± 3.12
Co2+	100	87.00 ± 2.76	128.00 ± 3.14	129.00 ± 4.49	61.00 ± 2.18
Mg2+	100	24.00 ± 9.50	37.00 ± 8.09	18.00 ± 1.43	16.00 ± 1.09
Mn2+	100	32.00 ± 1.80	41.00 ± 1.04	34.00 ± 1.04	38.00 ± 1.33

^aThe control protease activity corresponds to 100% activity in the absence of inhibitor. Values represent the means of three replicates.