Table 1.

FAE-gene targeted primer sets designed and employed in this study

Primer set	Annealing temp (°C)	Primer, positiona	Sequence (5′–3′)	Targeted FAE lineagesb
bssAc	58/52	7772f	GAC ATG ACC GAC GCS ATY CT	bssA sensu lato
		8546r	TC GTC GTC RTT GCC CCA YTT
FAE-B	58	7768f	C AAY GAT TTA ACC RAC GCC AT	Clostridial bssA, bssA sensu lato, nmsA
		8543r	TC GTC RTT GCC CCA YTT NGG
FAE-N	58	7363f	TC GCC GAG AAT TTC GAY TTG	nmsA sensu stricto
		7374f	TTC GAY TTG AGC GAC AGC GT
		8543r	TC GTC RTT GCC CCA YTT NGG
FAE-KM	58	FAE-Kf, 7757f-1	TCG GAC GCG TGC AAC GAT CTG A	assA
		FAE-Kf, 7757f-2	TCG GAC GCG TGC AAC GCC CTG A
		FAE-Mf, 7766f	TGT AAC GGC ATG ACC ATT GCG CT
		8543r	TC GTC RTT GCC CCA YTT NGG

^aThauera aromatica K127 bss operon position.

^bTargets intended in silico; see lineages in Fig. 1.

^cPrimer set of Winderl et al. (6, 15).