Table 2.
Experimentally tested performance of different FAE gene-targeted primers in selected strains and enrichments degrading hydrocarbons via fumarate addition
| Gene | FAE lineage | Culture | Substrate | Reference | Amplicon production with primer seta: |
|||
|---|---|---|---|---|---|---|---|---|
| bssA | FAE-B | FAE-N | FAE-KM | |||||
| bssA sensu lato | Proteobacteria | Azoarcus sp. T | Toluene | 37 | + | + | − | − |
| Geobacter metallireducens GS-15 | Toluene | 38 | + | − | − | − | ||
| Desulfotignum toluenicum H3 | Toluene | 39 | + | − | − | − | ||
| Clostridia | Desulfosporosinus sp. enrichment | Toluene | 15 | + | + | − | − | |
| Desulfitobacterium aromaticivorans UKTL | Toluene | 18 | − | + | − | − | ||
| Strain BF | Benzene | 19 | − | + | − | − | ||
| nmsA | Proteobacteria | Strain N47 | 2-Methyl-naphthalene | 21 | − | + | + | − |
| assA | Desulfatibacillum alkenivorans PF2803 | Alkanes | 40 | − | − | − | + | |
| Desulfatibacillum aliphaticivorans CV2803 | Alkanes | 41 | − | − | − | + | ||
a
+, a clear amplicon with the correct size was observed; −, no amplicon was obtained.