Table 4.

Sequencing-independent identification of T-RF lineages from Flingern sediment via dual-digest T-RFLP^a

Observed fragment size (bp)		In silico fragment sizeb (bp)		Candidate lineage	No. of FAE clones	Primer sets
TaqI	HaeIII	TaqI	HaeIII
364	398	362	397	Unidentified bssA homologues, unaffiliated (Testfeld Süd T1)	8	bssA, 52°C; bssA, 58°C; FAE-B
478	51	474	52	Deltaproteobacteria bssA, Desulfobulbaceae (F1 cluster)	14	bssA, 52°C; bssA, 58°C
678	304	670	300	Clostridial bssA Desulfosporosinus sp. related (F2 cluster)	22	bssA, 52°C
324	505	329	506	Deltaproteobacteria nmsA, Desulfobacteraceae (N47 related)	18	FAE-B, FAE-N
152	130	155	132	Clostridial bssA homologues, Desulfotomaculum related	1	FAE-N
	132	35	132	Betaproteobacteria bssA, Thauera sp. strain DNT-1	2	FAE-B
117	76	113	75	Deltaproteobacteria bssA, Desulfobacterium cetonicum bssA	1	bssA, 52°C

^aObserved T-RFs from the TaqI digest are also shown in Fig. 2; data for the HaeIII digest are not shown.

^bIn silico T-RFs are given for the labeled 8543r primer; hence, a difference of 3 bp must be considered for T-RFs delivered by the classical 8546r-labeled primer pair, as shown in Fig. 2.