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Influence of reaction pH on AA-2G synthesis by the wild-type and mutant CGTases with maltodextrin as the glycosyl donor. The pH 4.0 was 6.0 with acetic acid-sodium acetate buffer and 6.0 to 8.0 with phosphate buffer. The maximal AA-2G yield for the wild type and each mutant CGTase was defined as 100%.
