Fig 1.

Changes in intracellular free iron and corresponding changes in viability of Escherichia coli K-12 subjected to various ultrahigh-pressure (UHP) treatments (0.1 to 500 MPa for 1 min at 25 ± 2°C). (A) Iron electron paramagnetic resonance signals from a whole-cell preparation of E. coli subjected to various UHP treatments. (B) E. coli survivors (line) and amounts of free intracellular iron (hatched bars) after different pressure treatments. Error bars represent standard errors from three independent experiments.